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Design Specific Primers for qPCR [Step-by-Step Guide]

April 29, 2018 by Haseeb Ahmad Leave a Comment

Quantitative Polymerase Chain Reaction (a.k.a qPCR) is the way of detecting the amount of product obtained in the Polymerase Chain Reaction. Detecting the presence and measuring the amount of mRNA transcript in a particular part of an organism at a particular time is one of its best applications known as expression profilingĀ of genes. … [Read more...]

Filed Under: Science Tagged With: NCBI, PCR, Primer Designing, Primer-BLAST, Primers, Specific Primers

How to Get Better PCR Results (6 Pro Tips)

January 21, 2018 by Haseeb Ahmad Leave a Comment

Scientist with his PCR machine and test tubes for DNA Extraction

Are you struggling to get better PCR Results? You are not alone. Almost every early researcher working with DNA Amplification suffers something similar.   … [Read more...]

Filed Under: Science Tagged With: Biotechnology, Choose PCR Primer, dna, Lab tips, Laboratory, LabWeekly, PCR, PCR Cycles Guides, PCR Machine, PCR Temperature, Polymerase, Polymerase Chain Reaction, Primer Designing, Primers, Taq Polymerase, Thermocycler




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